Histamine H4 assay (GDH44)
| Type of assay | Biochemical |
| Receptor: | Mouse histamine H4 receptor |
| Expression system: | Transient transfection of HEK-293T |
| Radiotracer: | 0.1 nM [35S]GTPγS |
| Experiment settings: | - 8 concentrations of test compounds, each in triplicate – total reaction volume of 100 µL – 10 µM histamine (only for antagonism) – incubation at RT for 1 hours |
| Read-out: | Scintillation counting, converted in dpm unit |
| Reference agonist: | Histamine (EC50 = 265 nM) Thioperamide (IC50 = 373 nM at 10 µM histamine) |
| Z’ factor | >0.5 |
