Histamine H4 assay (GDH41)

human H4R binding
Type of assay Biochemical
Receptor: Human histamine H4 receptor
Expression system: Transient transfection of HEK-293T
Radioligand: [3H]histamine
Radioligand concentration: 7 nM
Kd (nM): 9 nM
Experiment settings: - 8 concentrations of test compounds, each in triplicate
– total reaction volume of 200 µL
– incubation at RT for 1 hour
Read-out: Scintillation counting, converted in dpm unit
Reference: JNJ 7777120 (pKi = 8.3)
Z’ factor >0.7