Histamine H4 assay (GDH41)
| Type of assay | Biochemical |
| Receptor: | Human histamine H4 receptor |
| Expression system: | Transient transfection of HEK-293T |
| Radioligand: | [3H]histamine |
| Radioligand concentration: | 7 nM |
| Kd (nM): | 9 nM |
| Experiment settings: | - 8 concentrations of test compounds, each in triplicate - total reaction volume of 200 µL - incubation at RT for 1 hour |
| Read-out: | Scintillation counting, converted in dpm unit |
| Reference: | JNJ 7777120 (pKi = 8.3) |
| Z’ factor | >0.7 |
