Histamine H3 assay (GDH35)

rat H3R binding

Type of assay Biochemical
Receptor: Rat histamine H3 receptor
Expression system: Transient transfection of HEK-293T
Radioligand: [3H]Nα-methylhistamine
Radioligand concentration: 0.5 nM
Kd (nM): 0.9 nM
Experiment settings: - 8 concentrations of test compounds, each in triplicate
– total reaction volume of 200 µL
– incubation at RT for 1 hour
Read-out: Scintillation counting, converted in dpm unit
Reference: Thioperamide (Ki = 20 nM)
Z’ factor >0.7