Histamine H2 assay (GDH21)

Type of assay Biochemical
Receptor: Human Histamine H2
Expression system: Transient transfection of HEK-293T
Radioligand: [3H]tiotidine
Radioligand concentration: 2 nM
Kd (nM): 12 nM
Experiment settings: - 8 concentrations of test compounds, each in triplicate
– total reaction volume of 200 µL
– incubation at RT for 1 hour
Read-out: Scintillation counting, converted in dpm unit
Reference: Ranitidine (Ki = 117 nM), tiotidine (Ki = 25 nM)
Z’ factor >0.7