Histamine H1 assay (GDH17)

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Type of assay Biochemical
Receptor: Guinea pig Histamine H1
Expression system: Transient transfection of HEK-293T
Radioligand: [3H]mepyramine
Radioligand concentration: 0.6 nM
Kd (nM): 1 nM
Experiment settings: - 8 concentrations of test compounds, each in triplicate
– total reaction volume of 200 µL
– incubation at RT for 1 hour
Read-out: Scintillation counting, converted in dpm unit
Reference: Mepyramine (pKi = 1 nM)
Z’ factor >0.7