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Type of assay | Cellular |
Receptor: | Rat histamine H1 |
Expression system: | Transient transfection of HEK-293 |
Reporter gene | NFAT-firefly luciferase |
Experiment settings: | - 8 concentrations of test compounds, each in triplicate – total reaction volume of 200 µL – total reaction volume of 200 µL – 1 µM histamine (only for antagonism) – incubation at 37°C for 6 hours |
Read-out: | Bioluminescence |
References: | Histamine (EC50 = 205 nM) Mepyramine (IC50 = 35 nM at 1 µM histamine) |
Z’ factor | >0.7 |