Histamine H1 assay (GDH16)

rat H1R functional
Type of assay Cellular
Receptor: Rat histamine H1
Expression system: Transient transfection of HEK-293
Reporter gene NFAT-firefly luciferase
Experiment settings: - 8 concentrations of test compounds, each in triplicate
– total reaction volume of 200 µL
– total reaction volume of 200 µL
– 1 µM histamine (only for antagonism)
– incubation at 37°C for 6 hours
Read-out: Bioluminescence
References: Histamine (EC50 = 205 nM)
Mepyramine (IC50 = 35 nM at 1 µM histamine)
Z’ factor >0.7