Histamine H1 assay (GDH14)
| Type of assay | Cellular |
| Receptor: | Mouse Histamine H1 |
| Expression system: | Transient transfection of HEK-293T |
| Reporter gene: | NFAT-firefly luciferase |
| Radioligand concentration: | 1.2 nM |
| Kd (nM): | 1 nM |
| Experiment settings: | - 8 concentrations of test compounds, each in triplicate – total reaction volume of 200 µL – 1 µM histamine (only for antagonism) – incubation at 37°C for 6 hours |
| Read-out: | Bioluminiscence |
| Reference: | Histamine (EC50 = 186 nM) Mepyramine (IC50 = 35 nM at 1 µM histamine) |
| Z’ factor | >0.7 |
