Histamine H1 assay (GDH11)

human H1R binding
Type of assay Biochemical
Receptor: Human Histamine H1
Expression system: Transient transfection of HEK-293T
Radioligand: [3H]mepyramine
Radioligand concentration: 1 nM
Kd (nM): 1 nM
Experiment settings: - 8 concentrations of test compounds, each in triplicate
– total reaction volume of 200 µL
– incubation at RT for 1 hour
Read-out: Scintillation counting, converted in dpm unit
Reference: Cetirizine (Ki = 8 nM)
Z’ factor >0.7