hERG binding assay (GDhERG)

Type of assay Biochemical
Receptor: human hERG
Expression system: Transient transfection of HEK-293T
Radioligand: [3H]astemizole
Radioligand concentration: 1 nM
Kd (nM): 1.3 nM
Experiment settings: - 8 concentrations of test compounds, each in triplicate
– total reaction volume of 100 µL
– incubation at RT for 1 hour
Read-out: Scintillation counting, converted in dpm unit
Reference: Astemizole (Ki = 2.8 nM)
Z’ factor >0.5