hERG binding assay (GDhERG)
| Type of assay | Biochemical |
| Receptor: | human hERG |
| Expression system: | Transient transfection of HEK-293T |
| Radioligand: | [3H]astemizole |
| Radioligand concentration: | 1 nM |
| Kd (nM): | 1.3 nM |
| Experiment settings: | - 8 concentrations of test compounds, each in triplicate – total reaction volume of 100 µL – incubation at RT for 1 hour |
| Read-out: | Scintillation counting, converted in dpm unit |
| Reference: | Astemizole (Ki = 2.8 nM) |
| Z’ factor | >0.5 |
